The Effect of Dietary Protein Source and Sulfur Amino acid Content on bone Metabolism in Growing Rats

This study was performed to evaluate the effect of dietary protein source and sulfur amino acid content on bone metabolism in rats. Thirty male rats (body weight 145 +/- 2 g) were divided into three groups. The rats in the first group were fed on casein 20% diet as animal protein source and those in the second group were fed on soy 20% diet as plant protein source. Sulfur amino acid ratio of these group was 1.07 : 1. The rats in the third group were fed on soy 20% diet and the sulfur amino acid were supplemented with the amount contained as much in the soy 20% diet. All rats were fed on experimental diet and deionized water ad libitum for 9 weeks, The total body, spine, femur bone mineral density and bone mineral content were measured using Dual Energy X-ray Absorptiometry. Calcium, phosphate, pyridinoline, creatinine in urine and calcium, phosphate, alkaline phosphatase, osteocalcin in serum were measured. During the experimental period, plant protein (soy protein) group had a lower urinary Ca excretion, urine pyridinoline & crosslinks value and had a higher Ca efficiency in total bone and femur bone mineral density than animal protein (casein) group. There were no significant differences in serum calcium, phosphate, alkaline phosphatase and osteocalcin among the three groups of the rats. The findings from this study demonstrated that plant protein (soy protein) is beneficial of bone mineral density because it had a higher Ca efficiency in total bone and femur bone mineral density than animal protein (casein). However, the supplementation of sulfur amino acid on soy results were consistent with prior studies that dietary sulfur amino acid load had a negative effect on calcium balance. The rats fed sulfur amino acid supplementation diet increased urinary calcium excretion and decreased calcium efficiency for total and femur mineral density. Therefore, dietary protein source and sulfur amino acid content influence bone metabolism.

Noninvasive detection of urinary sulfur for measuring the catabolism of sulfur amino acid / 中国微创外科杂志

Objective To test whether urinary sulfur excretion can be used as an accurate indicator of the catabolism of sulfur amino acid in growing newborn piglets. Methods Using a well-nourished enteral nutrition piglet model,we tested whether intravenous inorganic sulfate and methionine were fully excreted as sulfur in the urine. Results Recovery rate of inorganic sulfate and methionine as total sulfur in urine were 95.6% and 105.5%, respectively. Conclusions Detection of urinary sulfur, as a non-tracer and noninvasive method, may be employed to accurately measure the catabolism of sulfur amino acid in the growing piglet model.

Efeito dos níveis de metionina da dieta sobre o desempenho de poedeiras comerciais

The aim of this experiment was to evaluate the effects of addition of increasing levels of DL-methionine in diet on the performance of laying hens. Nine hundred and sisty Lohmann laying hens, randomly distributed into four treatments with four replicates were used. The control treatment (A) was not supplemented with DL-methionine and contained 0.29% of total methionine. The treatments B, C and D were supplemented with 0.03, 0.05 and 0.07% of methionine respectively, with total methionine being 0.32, 0.34 and 0.36%. The following characteristics were evaluated: egg production (%), feed intake (g), feed conversion (kg/dz), egg weight (g), egg mass (g/day) and eggshell thickness (mm). There were no differences (P>0.05) in terms of feed conversion and eggshell thickness among treatments. The treatments B, C and D produced better egg production, feed intake, egg mass and egg weight as compared to treatment A.

Avaliou-se o efeito da adição de níveis crescentes de DL-metionina na dieta sobre o desempenho de poedeiras comerciais. Foram utilizadas 960 poedeiras comerciais de linhagem Lohmann, distribuídas em quatro tratamentos, com quatro repetições de 60 aves cada, em um delineamento experimental inteiramente ao acaso. O tratamento A foi utilizado como controle, sem adição de DL-metionina, representando 0,29% do aminoácido na ração. Para os tratamentos B, C e D as rações foram suplementadas com 0,03, 0,05 e 0,07% de metionina, respectivamente, o que representou de 0,32, 0,34 e 0,36% do aminoácido na ração. Foram avaliadas as seguintes características: produção de ovos (%), consumo de ração (g), conversão alimentar (kg/dz), peso dos ovos (g), massa de ovo (g) e espessura da casca do ovo (mm). Não houve diferenças significativas (P 0,05) entre as médias para conversão alimentar e espessura da casca do ovo. Entretanto, para produção de ovos, peso dos ovos, massa de ovo e consumo de ração, os tratamentos suplementados com metionina apresentaram melhores resultados quando comparados ao tratamento sem suplementação.